RepeatExplorer2 includes utilities for Graph-based clustering and characterization of repetitive sequences in next-generation sequencing data and tools for the detection of transposable element protein coding domains.
How to user RepeatExplorer2:
- RepeatExplorer2 can be used through Galaxy based web interface on our public server at address https://galaxy-elixir.cerit-sc.cz. This server is provided as part of the Elixir CZ project and is maintained by CESNET and CERIT-SC that are participants of this project.
- Source code for local installation of RepeatExplorer2 can be found here. Basic documentation can be found in wiki.
Example of output from RepeatExplorer2:
Analysis of Pinus sylvestris genome can be found here
SeqGrapheR is a program for vizualization in exploration of sequence based graphs generated in RepeatExplorer output. SeqGrapheR program is now deposited in CRAN repository. See also our SeqGrapheR manual and input data examples.
The principles of RepeatExplorer approach are described in:
Novak, P., Neumann, P., Macas, J. (2010) – Graph-based clustering and characterization of repetitive sequences in next-generation sequencing data. BMC Bioinformatics 11: 378.
Novak, P., Neumann, P., Pech, J., Steinhaisl, J., Macas, J. (2013) – RepeatExplorer: a Galaxy-based web server for genome-wide characterization of eukaryotic repetitive elements from next generation sequence reads. Bioinformatics
Examples of RepeatExplorer can be found in:
Macas, J., Novak, P., Pellicer, J., Cizkova, J., Koblizkova, A., Neumann, P., Fukova, I., Dolezel, J., Kelly, L., Leitch, I. (2015) – In depth characterization of repetitive DNA in 23 plant genomes reveals sources of genome size variation in the legume tribe Fabeae. PLoS ONE 10: e0143424.
Dodsworth, S., Chase, M.W., Kelly, L.J., Leitch, I.J., Macas, J., Novak, P., Piednoël, M., Weiss-Schneeweiss, H., Leitch, A. (2015) – Genomic repeat abundances contain phylogenetic signal. Syst. Biol. 64: 112-126.
Novak, P., Hribova, E., Neumann, P., Koblizkova, A., Dolezel, J., Macas, J. (2014) – Genome-wide analysis of repeat diversity across the family Musaceae. PLoS ONE 9: e98918.